Hotspot

psbZ-trnG-UCC

LSC · 45550-46287 · 738 bp

Back to Robinia

Pi 0.0178

MarkerSeek score 64.90

Primer available yes

Label psbZ-trnG-UCC

Feature Metadata

label_name: psbZ-trnG-UCC
feature_type: igs
region: LSC
length_bp: 738
variable_sites: 10
pi: 0.0178
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.76
markerseek_score: 64.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbZ-trnG-UCC_p1	CTTCTCCTGATGGTTGGTCG	ATAAAAAGGCAGTGGCGGAT	55.0	45.0	58.0	57.9	782-929 bp	1.0	83.7
psbZ-trnG-UCC_p2	CTTCTCCTGATGGTTGGTCG	CGTCTTCTCCTTGGCAAAGA	55.0	50.0	58.0	57.8	745-892 bp	1.0	83.5
psbZ-trnG-UCC_p3	TTGCTTCTCCTGATGGTTGG	ATAAAAAGGCAGTGGCGGAT	50.0	45.0	57.8	57.9	785-932 bp	1.0	82.1
psbZ-trnG-UCC_p4	TCCTGATGGTTGGTCGAGTA	ATAAAAAGGCAGTGGCGGAT	50.0	45.0	57.8	57.9	778-925 bp	1.0	82.0
psbZ-trnG-UCC_p5	TTGCTTCTCCTGATGGTTGG	CGTCTTCTCCTTGGCAAAGA	50.0	50.0	57.8	57.8	748-895 bp	1.0	82.0