Hotspot

atpH-atpI

LSC · 22108-23490 · 1383 bp

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Pi 0.0154

MarkerSeek score 55.00

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 1383
variable_sites: 21
pi: 0.0154
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 55.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	ATAACGGAAGCGGCAGAAAT	GAATATGGTCCGTGGGTTCC	45.0	55.0	58.0	58.0	1892-1927 bp	1.0	81.3
atpH-atpI_p2	ATAACGGAAGCGGCAGAAAT	ATTGGTCAGGGGCTCTTTTG	45.0	50.0	58.0	58.1	1834-1869 bp	1.0	81.1
atpH-atpI_p3	ATAACGGAAGCGGCAGAAAT	CGCAAACCATTCCCACTTTC	45.0	50.0	58.0	57.9	1978-2013 bp	1.0	81.0
atpH-atpI_p4	ATAACGGAAGCGGCAGAAAT	GCAAACCATTCCCACTTTCG	45.0	50.0	58.0	57.9	1977-2012 bp	1.0	81.0
atpH-atpI_p5	ATAACGGAAGCGGCAGAAAT	CGAAATCCGCAAACCATTCC	45.0	50.0	58.0	58.1	1985-2020 bp	1.0	80.8