Hotspot

trnS-GGA-trnG-GCC

LSC · 16173-16930 · 758 bp

Back to Robinia

Pi 0.0218
MarkerSeek score 69.10
Primer available yes
Label trnS-GGA-trnG-GCC

Feature Metadata

label_name
trnS-GGA-trnG-GCC
feature_type
igs
region
LSC
length_bp
758
variable_sites
16
pi
0.0218
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.97
markerseek_score
69.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GGA-trnG-GCC_p1 CAATCCGACGCTTTAGTCCA GGGTCTATACAACGAATCGCA 50.0 47.6 58.0 57.9 818-838 bp 1.0 75.2
trnS-GGA-trnG-GCC_p2 AATCCGACGCTTTAGTCCAC GGGTCTATACAACGAATCGCA 50.0 47.6 58.0 57.9 817-837 bp 1.0 75.2
trnS-GGA-trnG-GCC_p3 CAATCCGACGCTTTAGTCCA ACGAATCGCACTTTTACCACT 50.0 42.9 58.0 58.2 807-827 bp 1.0 74.1
trnS-GGA-trnG-GCC_p4 AATCCGACGCTTTAGTCCAC ACGAATCGCACTTTTACCACT 50.0 42.9 58.0 58.2 806-826 bp 1.0 74.1
trnS-GGA-trnG-GCC_p5 CAATCCGACGCTTTAGTCCA ACGAATCGCACTTTTACCAC 50.0 45.0 58.0 56.8 807-827 bp 1.0 74.0