Hotspot

rps4-trnS-GGA

LSC · 15857-16085 · 229 bp

Back to Robinia

Pi 0.0175
MarkerSeek score 52.70
Primer available yes
Label rps4-trnS-GGA

Feature Metadata

label_name
rps4-trnS-GGA
feature_type
igs
region
LSC
length_bp
229
variable_sites
4
pi
0.0175
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
52.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps4-trnS-GGA_p1 GCTGGTTGTAGAATATTATTCTCGC TGGACTAAAGCGTCGGATTG 40.0 50.0 58.2 58.0 335 bp 1.0 41.0
rps4-trnS-GGA_p2 TGGTTGTAGAATATTATTCTCGCCA TGGACTAAAGCGTCGGATTG 36.0 50.0 58.2 58.0 333 bp 1.0 41.0
rps4-trnS-GGA_p3 GCTGGTTGTAGAATATTATTCTCGC GTGGACTAAAGCGTCGGATT 40.0 50.0 58.2 58.0 336 bp 1.0 41.0
rps4-trnS-GGA_p4 TGGTTGTAGAATATTATTCTCGCCA GTGGACTAAAGCGTCGGATT 36.0 50.0 58.2 58.0 334 bp 1.0 41.0
rps4-trnS-GGA_p5 TGAATTGCTGGTTGTAGAAT TGGACTAAAGCGTCGGATTG 35.0 50.0 52.8 58.0 341 bp 1.0 40.9