Hotspot

rpl16

LSC · 85390-86872 · 1483 bp

Back to Ripogonum

Pi 0.00274

MarkerSeek score 35.10

Primer available yes

Label rpl16

Feature Metadata

label_name: rpl16
feature_type: gene
region: LSC
length_bp: 1483
variable_sites: 11
pi: 0.00274
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 35.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16_p1	CGCACCAAAAACTCGAGTTC	GGTTTCGAAGGAATTGCACG	50.0	50.0	58.0	58.0	2555-2565 bp	1.0	80.9
rpl16_p2	ATCAATTTTCGAGCTCCGCT	GGTTTCGAAGGAATTGCACG	45.0	50.0	58.0	58.0	2311-2321 bp	1.0	80.9
rpl16_p3	CGCACCAAAAACTCGAGTTC	CGCGGGTATACAGGAAGATG	50.0	55.0	58.0	57.9	2631-2641 bp	1.0	80.6
rpl16_p4	ATCAATTTTCGAGCTCCGCT	CGCGGGTATACAGGAAGATG	45.0	55.0	58.0	57.9	2387-2397 bp	1.0	80.6
rpl16_p5	CGCACCAAAAACTCGAGTTC	TAAGAGAACGTCCTCGGGTT	50.0	50.0	58.0	58.1	2571-2581 bp	1.0	80.5