Hotspot

petD

LSC · 80681-81964 · 1284 bp

Back to Ripogonum

Pi 0.00202

MarkerSeek score 38.00

Primer available yes

Label petD

Feature Metadata

label_name: petD
feature_type: gene
region: LSC
length_bp: 1284
variable_sites: 6
pi: 0.00202
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 38.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petD_p1	AGTTGAAGTGGATTCTCCGA	TTCATCTTCAGCACGATCCG	45.0	50.0	56.2	58.1	1425-1431 bp	1.0	65.2
petD_p2	TGAAGTGGATTCTCCGAAGAG	TTCATCTTCAGCACGATCCG	47.6	50.0	57.1	58.1	1422-1428 bp	1.0	64.7
petD_p3	AGTTGAAGTGGATTCTCCGA	CTTCAGCACGATCCGTACTC	45.0	55.0	56.2	58.2	1420-1426 bp	1.0	64.4
petD_p4	TGAAGTGGATTCTCCGAAGAG	CTTCAGCACGATCCGTACTC	47.6	55.0	57.1	58.2	1417-1423 bp	1.0	64.0
petD_p5	AGTTGAAGTGGATTCTCCGAA	TTCATCTTCAGCACGATCCG	42.9	50.0	57.0	58.1	1425-1431 bp	1.0	63.4