Hotspot

ndhA

SSC · 125415-127576 · 2162 bp

Back to Ripogonum

Pi 0.00204

MarkerSeek score 52.70

Primer available yes

Label ndhA

Feature Metadata

label_name: ndhA
feature_type: gene
region: SSC
length_bp: 2162
variable_sites: 10
pi: 0.00204
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhA_p1	TGAACCCAGTCACCATAGGA	CGCCCGGTTTCATCAATTTG	50.0	50.0	57.7	58.4	2391-2395 bp	1.0	74.7
ndhA_p2	TGAACCCAGTCACCATAGGA	GCCCGGTTTCATCAATTTGC	50.0	50.0	57.7	58.6	2390-2394 bp	1.0	72.6
ndhA_p3	TGAACCCAGTCACCATAGGA	CCCGGTTTCATCAATTTGCA	50.0	45.0	57.7	57.3	2389-2393 bp	1.0	71.8
ndhA_p4	TGAACCCAGTCACCATAGGA	CCCGGTTTCATCAATTTGCAA	50.0	42.9	57.7	58.0	2389-2393 bp	1.0	69.4
ndhA_p5	CATGAACCCAGTCACCATAGG	CGCCCGGTTTCATCAATTTG	52.4	50.0	58.1	58.4	2393-2397 bp	1.0	68.8