Hotspot

rps15-ycf1

SSC · 129138-129534 · 397 bp

Back to Ripogonum

Pi 0.016

MarkerSeek score 46.40

Primer available yes

Label rps15-ycf1

Feature Metadata

label_name: rps15-ycf1
feature_type: igs
region: SSC
length_bp: 397
variable_sites: 12
pi: 0.016
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 46.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15-ycf1_p1	TCAACAGACCCTTTGTTTTCT	CGGCAGTCGTTTCAGTATGT	38.1	50.0	55.5	58.3	523-525 bp	1.0	51.9
rps15-ycf1_p2	TCAACAGACCCTTTGTTTTCTTC	CGGCAGTCGTTTCAGTATGT	39.1	50.0	57.4	58.3	523-525 bp	1.0	50.9
rps15-ycf1_p3	CAACAGACCCTTTGTTTTCTTCT	CGGCAGTCGTTTCAGTATGT	39.1	50.0	57.4	58.3	522-524 bp	1.0	50.9
rps15-ycf1_p4	TGAAATTCAACAGACCCTTTGT	CGGCAGTCGTTTCAGTATGT	36.4	50.0	56.4	58.3	529-531 bp	1.0	50.5
rps15-ycf1_p5	ACTTGAAATTCAACAGACCCT	CGGCAGTCGTTTCAGTATGT	38.1	50.0	55.3	58.3	532-534 bp	1.0	50.0