Hotspot

rpl20-rps12

LSC · 72939-73704 · 766 bp

Back to Ripogonum

Pi 0.00131

MarkerSeek score 55.40

Primer available yes

Label rpl20-rps12

Feature Metadata

label_name: rpl20-rps12
feature_type: igs
region: LSC
length_bp: 766
variable_sites: 3
pi: 0.00131
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 55.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl20-rps12_p1	GTCTCCGAGCTGTATACCCT	GAAATCCCCCGCTCTTAGAG	55.0	55.0	58.0	57.5	852-861 bp	1.0	77.1
rpl20-rps12_p2	GTCTCCGAGCTGTATACCCT	AAATGTCACGAAATCCCCCG	55.0	50.0	58.0	58.5	861-870 bp	1.0	77.0
rpl20-rps12_p3	AGTCTTGAATGAGCCCCTCT	GAAATCCCCCGCTCTTAGAG	50.0	55.0	58.0	57.5	907-916 bp	1.0	76.8
rpl20-rps12_p4	TGAGCCCCTCTAAAGGTTGA	GAAATCCCCCGCTCTTAGAG	50.0	55.0	58.0	57.5	898-907 bp	1.0	76.8
rpl20-rps12_p5	AGTCTTGAATGAGCCCCTCT	AAATGTCACGAAATCCCCCG	50.0	50.0	58.0	58.5	916-925 bp	1.0	76.8