Hotspot

petA-psbJ

LSC · 66461-67632 · 1172 bp

Back to Ripogonum

Pi 0.00262

MarkerSeek score 62.10

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 1172
variable_sites: 7
pi: 0.00262
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 62.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	GGCATCCGTTATTTTGGCAC	GGTTCATATTCCGGGTTGGG	50.0	55.0	58.1	58.3	1282-1292 bp	1.0	78.3
petA-psbJ_p2	GGCCTTTTGTTCTTCTTGGC	GGTTCATATTCCGGGTTGGG	50.0	55.0	57.8	58.3	1299-1309 bp	1.0	77.7
petA-psbJ_p3	GGCATCCGTTATTTTGGCAC	CATATTCCGGGTTGGGTTCA	50.0	50.0	58.1	57.6	1278-1288 bp	1.0	77.4
petA-psbJ_p4	GGCATCCGTTATTTTGGCAC	GTTCATATTCCGGGTTGGGT	50.0	50.0	58.1	57.6	1281-1291 bp	1.0	77.4
petA-psbJ_p5	TGTTCTTCTTGGCATCCGTT	GGTTCATATTCCGGGTTGGG	45.0	55.0	57.7	58.3	1292-1302 bp	1.0	76.8