Hotspot

ndhK-ndhC

LSC · 52494-52549 · 56 bp

Back to Ripogonum

Pi 0.0845

MarkerSeek score 59.00

Primer available yes

Label ndhK-ndhC

Feature Metadata

label_name: ndhK-ndhC
feature_type: igs
region: LSC
length_bp: 56
variable_sites: 8
pi: 0.0845
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhK-ndhC_p1	ACGGAAACTCAATCAAATTCA	GGTTCAGTTTATGCATGGCG	33.3	50.0	54.0	57.8	123 bp	1.0	45.2
ndhK-ndhC_p2	CAAGTAACGGAAACTCAATCA	GGTTCAGTTTATGCATGGCG	38.1	50.0	54.2	57.8	129 bp	1.0	45.0
ndhK-ndhC_p3	CAAGTAACGGAAACTCAATCAA	GGTTCAGTTTATGCATGGCG	36.4	50.0	55.0	57.8	129 bp	1.0	45.0
ndhK-ndhC_p4	ACGGAAACTCAATCAAATTCA	TGGGCAATGAGTTTCGATGT	33.3	45.0	54.0	57.8	198 bp	1.0	43.2
ndhK-ndhC_p5	CAAGTAACGGAAACTCAATCA	TGGGCAATGAGTTTCGATGT	38.1	45.0	54.2	57.8	204 bp	1.0	43.1