Hotspot

trnE_UUC-trnT_GGU

LSC · 32893-33657 · 765 bp

Back to Ripogonum

Pi 0.00392

MarkerSeek score 52.50

Primer available yes

Label trnE_UUC-trnT_GGU

Feature Metadata

label_name: trnE_UUC-trnT_GGU
feature_type: igs
region: LSC
length_bp: 765
variable_sites: 7
pi: 0.00392
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 52.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnE_UUC-trnT_GGU_p1	AGATGTCCTGAACCGCTAGA	GAACCGATGACTTACGCCTT	50.0	50.0	57.9	58.0	824-850 bp	1.0	82.1
trnE_UUC-trnT_GGU_p2	AGAGATGTCCTGAACCGCTA	GAACCGATGACTTACGCCTT	50.0	50.0	57.9	58.0	826-852 bp	1.0	82.0
trnE_UUC-trnT_GGU_p3	TTTCGTAGTACCCTACCCCC	GAACCGATGACTTACGCCTT	55.0	50.0	57.8	58.0	880-906 bp	1.0	81.7
trnE_UUC-trnT_GGU_p4	AGAGATGTCCTGAACCGCTA	CCATGGCGTTACTCTACCAC	50.0	55.0	57.9	58.1	805-831 bp	1.0	81.7
trnE_UUC-trnT_GGU_p5	AGATGTCCTGAACCGCTAGA	CCATGGCGTTACTCTACCAC	50.0	55.0	57.9	58.1	803-829 bp	1.0	81.7