Hotspot

petN-psbM

LSC · 30260-31119 · 860 bp

Back to Ripogonum

Pi 0.00628
MarkerSeek score 58.00
Primer available yes
Label petN-psbM

Feature Metadata

label_name
petN-psbM
feature_type
igs
region
LSC
length_bp
860
variable_sites
12
pi
0.00628
unique_haplotype_count
5
primer_available
yes
alignment_reliability
0.99
markerseek_score
58.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petN-psbM_p1 AGTATGGGGAAGGAGTGGAC TCATTCTAGTTCCTACCGCCT 55.0 47.6 58.1 58.0 950-1042 bp 1.0 72.4
petN-psbM_p2 GTATGGGGAAGGAGTGGACT TCATTCTAGTTCCTACCGCCT 55.0 47.6 58.1 58.0 949-1041 bp 1.0 72.4
petN-psbM_p3 TAGTAAGTCTCGCTTGGGCT TCATTCTAGTTCCTACCGCCT 50.0 47.6 58.2 58.0 1008-1100 bp 1.0 72.1
petN-psbM_p4 TATGGGGAAGGAGTGGACTC TCATTCTAGTTCCTACCGCCT 55.0 47.6 57.8 58.0 948-1040 bp 1.0 72.0
petN-psbM_p5 GGGGAAGGAGTGGACTCTAG TCATTCTAGTTCCTACCGCCT 60.0 47.6 58.2 58.0 945-1037 bp 1.0 71.7