Hotspot

trnC_GCA-petN

LSC · 29038-30169 · 1132 bp

Back to Ripogonum

Pi 0.0023

MarkerSeek score 55.70

Primer available yes

Label trnC_GCA-petN

Feature Metadata

label_name: trnC_GCA-petN
feature_type: igs
region: LSC
length_bp: 1132
variable_sites: 6
pi: 0.0023
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC_GCA-petN_p1	AGTTCAAATCTGGGTGTCGC	AGTCCACTCCTTCCCCATAC	50.0	55.0	58.5	58.1	1124-1241 bp	1.0	79.6
trnC_GCA-petN_p2	AGTTCAAATCTGGGTGTCGC	GTCCACTCCTTCCCCATACT	50.0	55.0	58.5	58.1	1123-1240 bp	1.0	79.6
trnC_GCA-petN_p3	AGTTCAAATCTGGGTGTCGC	AGCCCAAGCGAGACTTACTA	50.0	50.0	58.5	58.2	1065-1182 bp	1.0	79.4
trnC_GCA-petN_p4	GGGGGACTGCAAATCCTTTA	AGTCCACTCCTTCCCCATAC	50.0	55.0	57.5	58.1	1149-1266 bp	1.0	79.2
trnC_GCA-petN_p5	GGGGGACTGCAAATCCTTTA	GTCCACTCCTTCCCCATACT	50.0	55.0	57.5	58.1	1148-1265 bp	1.0	79.2