Hotspot

rpoB-trnC_GCA

LSC · 27987-28966 · 980 bp

Back to Ripogonum

Pi 0.00548

MarkerSeek score 51.50

Primer available yes

Label rpoB-trnC_GCA

Feature Metadata

label_name: rpoB-trnC_GCA
feature_type: igs
region: LSC
length_bp: 980
variable_sites: 13
pi: 0.00548
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 51.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC_GCA_p1	GTGGACATCCCCTCATTTCC	CCCAATTTGTTGATCAGGCG	55.0	50.0	57.9	57.7	1094-1098 bp	1.0	78.7
rpoB-trnC_GCA_p2	GTGGACATCCCCTCATTTCC	GCGACACCCAGATTTGAACT	55.0	50.0	57.9	58.5	1077-1081 bp	1.0	77.2
rpoB-trnC_GCA_p3	GTGGACATCCCCTCATTTCC	TAAAGGATTTGCAGTCCCCC	55.0	50.0	57.9	57.5	1052-1056 bp	1.0	76.8
rpoB-trnC_GCA_p4	CATCCCCTCATTTCCCTTCC	CCCAATTTGTTGATCAGGCG	55.0	50.0	57.6	57.7	1089-1093 bp	1.0	76.2
rpoB-trnC_GCA_p5	GTGGACATCCCCTCATTTCC	TTTGTTGATCAGGCGACACC	55.0	50.0	57.9	58.8	1089-1093 bp	1.0	74.9