Hotspot

atpF-atpH

LSC · 14104-14617 · 514 bp

Back to Ripogonum

Pi 0.00473

MarkerSeek score 59.00

Primer available yes

Label atpF-atpH

Feature Metadata

label_name: atpF-atpH
feature_type: igs
region: LSC
length_bp: 514
variable_sites: 6
pi: 0.00473
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 59.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF-atpH_p1	TTAAACCCGAAACTCCCTGC	AATTTACGGACTGGTCGTGG	50.0	50.0	58.1	57.9	626-638 bp	1.0	80.6
atpF-atpH_p2	CCAATAGCCCAAGGAAACGA	AATTTACGGACTGGTCGTGG	50.0	50.0	57.9	57.9	600-612 bp	1.0	80.5
atpF-atpH_p3	TTAAACCCGAAACTCCCTGC	GCGGAGGGTAAAATACGAGG	50.0	55.0	58.1	58.1	685-697 bp	1.0	80.1
atpF-atpH_p4	CCAATAGCCCAAGGAAACGA	GCGGAGGGTAAAATACGAGG	50.0	55.0	57.9	58.1	659-671 bp	1.0	80.0
atpF-atpH_p5	TTAAACCCGAAACTCCCTGC	TCGTGGCATTAGCGCTTTTA	50.0	45.0	58.1	58.3	612-624 bp	1.0	79.2