Hotspot

ycf1

SSC · 123601-128991 · 5391 bp

Back to Ricotia

Pi 0.0283

MarkerSeek score 59.00

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5391
variable_sites: 285
pi: 0.0283
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	AACGTGGTTTGTTGCAACTA	TGCGTTCCCAAAGACGTAAA	40.0	45.0	56.4	58.0	1095-1110 bp	0.75	62.3
ycf1_p2	TAACGTGGTTTGTTGCAACT	TGCGTTCCCAAAGACGTAAA	40.0	45.0	56.4	58.0	1096-1111 bp	0.75	62.3
ycf1_p3	AACGTGGTTTGTTGCAACTA	TTAAAGGGTCCATGCGTTCC	40.0	50.0	56.4	58.2	1107-1122 bp	0.75	61.3
ycf1_p4	TAACGTGGTTTGTTGCAACT	TTAAAGGGTCCATGCGTTCC	40.0	50.0	56.4	58.2	1108-1123 bp	0.75	61.3
ycf1_p5	AACGTGGTTTGTTGCAACTA	ATGCGTTCCCAAAGACGTAA	40.0	45.0	56.4	57.8	1096-1111 bp	0.75	61.3