Hotspot

trnL-ycf5

SSC · 114039-114152 · 114 bp

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Pi 0.0556

MarkerSeek score 83.40

Primer available yes

Label trnL-ycf5

Feature Metadata

label_name: trnL-ycf5
feature_type: igs
region: SSC
length_bp: 114
variable_sites: 12
pi: 0.0556
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 83.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-ycf5_p1	TAGACACGCTGCTCTTAGGA	TCAGTACAATTGAAACGACCGA	50.0	40.9	57.9	58.0	237-243 bp	1.0	69.8
trnL-ycf5_p2	TAGACACGCTGCTCTTAGGA	GTCAGTACAATTGAAACGACCG	50.0	45.5	57.9	58.0	238-244 bp	1.0	69.6
trnL-ycf5_p3	AAGTTGCCGCTATGGTGAAA	TCAGTACAATTGAAACGACCGA	45.0	40.9	58.1	58.0	261-267 bp	1.0	69.4
trnL-ycf5_p4	AAGTTGCCGCTATGGTGAAA	GTCAGTACAATTGAAACGACCG	45.0	45.5	58.1	58.0	262-268 bp	1.0	69.2
trnL-ycf5_p5	AGTTGCCGCTATGGTGAAAT	TCAGTACAATTGAAACGACCGA	45.0	40.9	57.9	58.0	260-266 bp	1.0	69.2