Hotspot

rpl32-trnL

SSC · 113043-113956 · 914 bp

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Pi 0.0491

MarkerSeek score 81.30

Primer available yes

Label rpl32-trnL

Feature Metadata

label_name: rpl32-trnL
feature_type: igs
region: SSC
length_bp: 914
variable_sites: 79
pi: 0.0491
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 81.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL_p1	AAATGAGCACTCTCGTACGG	TCCTAAGAGCAGCGTGTCTA	50.0	50.0	57.7	57.9	1240-1359 bp	1.0	86.9
rpl32-trnL_p2	AAATGAGCACTCTCGTACGG	TAACCACTGGGACTGGAGAG	50.0	55.0	57.7	58.1	1813-1926 bp	1.0	86.7
rpl32-trnL_p3	ATGAGCACTCTCGTACGGAT	TCCTAAGAGCAGCGTGTCTA	50.0	50.0	58.3	57.9	1238-1357 bp	1.0	86.6
rpl32-trnL_p4	ATGAGCACTCTCGTACGGAT	TAACCACTGGGACTGGAGAG	50.0	55.0	58.3	58.1	1811-1924 bp	1.0	86.4
rpl32-trnL_p5	AAATGAGCACTCTCGTACGG	TTGGCGTAGTTAGTGCGTTT	50.0	45.0	57.7	58.1	1801 bp	0.25	46.7