Hotspot

ndhF-rpl32

SSC · 112036-112883 · 848 bp

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Pi 0.0427
MarkerSeek score 79.70
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
848
variable_sites
64
pi
0.0427
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.96
markerseek_score
79.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 GGGATGATCCATGAATATTGA ACGAATACGCTTTTTCGAGA 38.1 40.0 52.8 55.5 899-931 bp 1.0 49.6
ndhF-rpl32_p2 GGGATGATCCATGAATATTGAT ACGAATACGCTTTTTCGAGA 36.4 40.0 53.5 55.5 899-931 bp 1.0 49.6
ndhF-rpl32_p3 GGGATGATCCATGAATATTGA ACGAATACGCTTTTTCGAGAT 38.1 38.1 52.8 56.1 899-931 bp 1.0 49.6
ndhF-rpl32_p4 GGGATGATCCATGAATATTGAT ACGAATACGCTTTTTCGAGAT 36.4 38.1 53.5 56.1 899-931 bp 1.0 49.6
ndhF-rpl32_p5 GGGATGATCCATGAATATTGATATGT ACGAATACGCTTTTTCGAGA 34.6 40.0 57.0 55.5 899-931 bp 1.0 49.6