Hotspot

petD-rpoA

LSC · 76968-77172 · 205 bp

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Pi 0.0504

MarkerSeek score 76.90

Primer available yes

Label petD-rpoA

Feature Metadata

label_name: petD-rpoA
feature_type: igs
region: LSC
length_bp: 205
variable_sites: 20
pi: 0.0504
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 76.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petD-rpoA_p1	GCCCTGTGGTTAGGTATTGG	GAAGCCTCCCGGAATTTGAT	55.0	50.0	57.9	57.9	605-608 bp	1.0	83.2
petD-rpoA_p2	GGCCCTGTGGTTAGGTATTG	GAAGCCTCCCGGAATTTGAT	55.0	50.0	57.9	57.9	606-609 bp	1.0	83.2
petD-rpoA_p3	GCCCTGTGGTTAGGTATTGG	TAATTTTACCGCCCTCCGTG	55.0	50.0	57.9	58.0	894 bp	0.25	49.4
petD-rpoA_p4	GGCCCTGTGGTTAGGTATTG	TAATTTTACCGCCCTCCGTG	55.0	50.0	57.9	58.0	895 bp	0.25	49.4
petD-rpoA_p5	CCGTCTTTTTGATTGGCACC	TAATTTTACCGCCCTCCGTG	50.0	50.0	57.9	58.0	920 bp	0.25	49.3