Hotspot

trnP-psaJ

LSC · 65785-66118 · 334 bp

Back to Ricotia

Pi 0.0334

MarkerSeek score 67.30

Primer available yes

Label trnP-psaJ

Feature Metadata

label_name: trnP-psaJ
feature_type: igs
region: LSC
length_bp: 334
variable_sites: 20
pi: 0.0334
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 67.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnP-psaJ_p1	ACCCGTGACATTTTGTACCC	AAACCTGCTAACGAACCGAA	50.0	45.0	58.1	57.8	463-466 bp	1.0	80.9
trnP-psaJ_p2	CCCGTGACATTTTGTACCCA	AAACCTGCTAACGAACCGAA	50.0	45.0	58.1	57.8	462-465 bp	1.0	80.9
trnP-psaJ_p3	ACAGGATTTGAACCCGTGAC	AAACCTGCTAACGAACCGAA	50.0	45.0	58.1	57.8	474-477 bp	1.0	80.7
trnP-psaJ_p4	CAGGATTTGAACCCGTGACA	AAACCTGCTAACGAACCGAA	50.0	45.0	58.1	57.8	473-476 bp	1.0	80.7
trnP-psaJ_p5	TGACAGGATTTGAACCCGTG	AAACCTGCTAACGAACCGAA	50.0	45.0	58.1	57.8	476-479 bp	1.0	80.7