Hotspot

ORF31-petG

LSC · 65009-65205 · 197 bp

Back to Ricotia

Pi 0.0372

MarkerSeek score 73.30

Primer available yes

Label ORF31-petG

Feature Metadata

label_name: ORF31-petG
feature_type: igs
region: LSC
length_bp: 197
variable_sites: 13
pi: 0.0372
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 73.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ORF31-petG_p1	TGGTTTTCTACTAGCAGCTT	CAACTGATCACCACGTCTGT	40.0	50.0	54.3	57.8	364-375 bp	1.0	54.5
ORF31-petG_p2	TTGGTTTTCTACTAGCAGCT	CAACTGATCACCACGTCTGT	40.0	50.0	54.3	57.8	365-376 bp	1.0	54.4
ORF31-petG_p3	TGGTTTTCTACTAGCAGCTTT	CAACTGATCACCACGTCTGT	38.1	50.0	55.1	57.8	364-375 bp	1.0	53.3
ORF31-petG_p4	TTGGTTTTCTACTAGCAGCTT	CAACTGATCACCACGTCTGT	38.1	50.0	55.1	57.8	365-376 bp	1.0	53.3
ORF31-petG_p5	TTTGGTTTTCTACTAGCAGCT	CAACTGATCACCACGTCTGT	38.1	50.0	55.1	57.8	366-377 bp	1.0	53.3