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psbE-ORF31

LSC · 63538-64912 · 1375 bp

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Pi 0.0449

MarkerSeek score 78.00

Primer available yes

Label psbE-ORF31

Feature Metadata

label_name: psbE-ORF31
feature_type: igs
region: LSC
length_bp: 1375
variable_sites: 107
pi: 0.0449
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 78.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-ORF31_p1	CCCAAACACGTCGTAAGCTA	AGCGCTCTTAGTTCAGTTCG	50.0	50.0	57.9	58.0	1702-2139 bp	1.0	88.4
psbE-ORF31_p2	CGGTAGGTACAGCTAGTCCA	AGCGCTCTTAGTTCAGTTCG	55.0	50.0	58.0	58.0	1892-2329 bp	1.0	88.2
psbE-ORF31_p3	ACGGTAGGTACAGCTAGTCC	AGCGCTCTTAGTTCAGTTCG	55.0	50.0	58.0	58.0	1893-2330 bp	1.0	88.1
psbE-ORF31_p4	TCAGCCATTTATCCCGCTTC	AGCGCTCTTAGTTCAGTTCG	50.0	50.0	58.0	58.0	2232-2668 bp	1.0	88.0
psbE-ORF31_p5	TCAGCCATTTATCCCGCTTC	AGAACGTGGGTCTCCAAAAC	50.0	50.0	58.0	58.0	2210-2646 bp	1.0	87.9