Hotspot

trnM-atpE

LSC · 51385-51531 · 147 bp

Back to Ricotia

Pi 0.0509

MarkerSeek score 79.50

Primer available yes

Label trnM-atpE

Feature Metadata

label_name: trnM-atpE
feature_type: igs
region: LSC
length_bp: 147
variable_sites: 13
pi: 0.0509
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 79.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnM-atpE_p1	GGCAGGAGTCATTGGTTCAA	AGTCGAGGCTCTCAATACGA	50.0	50.0	58.1	58.0	201-207 bp	1.0	86.8
trnM-atpE_p2	GGCAGGAGTCATTGGTTCAA	ACGAGTCGAGGCTCTCAATA	50.0	50.0	58.1	58.0	204-210 bp	1.0	86.7
trnM-atpE_p3	GGCAGGAGTCATTGGTTCAA	CTCTCAGACGAGCTAGGACA	50.0	55.0	58.1	58.0	223-229 bp	1.0	86.1
trnM-atpE_p4	TTGCTTTCATACGGCAGGAG	AGTCGAGGCTCTCAATACGA	50.0	50.0	58.3	58.0	213-219 bp	1.0	85.0
trnM-atpE_p5	TTGCTTTCATACGGCAGGAG	CTCTCAGACGAGCTAGGACA	50.0	55.0	58.3	58.0	235-241 bp	1.0	84.4