Hotspot

psbG-ndhC

LSC · 49293-49356 · 64 bp

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Pi 0.0781

MarkerSeek score 78.70

Primer available yes

Label psbG-ndhC

Feature Metadata

label_name: psbG-ndhC
feature_type: igs
region: LSC
length_bp: 64
variable_sites: 8
pi: 0.0781
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 78.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbG-ndhC_p1	GGCTGGAAAGTCTTGACCAA	TCGATGTACTGGGGGTATCC	50.0	55.0	58.0	58.0	271 bp	0.5	61.6
psbG-ndhC_p2	GTACCATAAAGAAGCGGCCA	TCGATGTACTGGGGGTATCC	50.0	55.0	58.0	58.0	293 bp	0.5	61.6
psbG-ndhC_p3	TGGTACCATAAAGAAGCGGC	TCGATGTACTGGGGGTATCC	50.0	55.0	58.0	58.0	295 bp	0.5	61.6
psbG-ndhC_p4	ATCAACTCCGCGAACAGTAC	TCGATGTACTGGGGGTATCC	50.0	55.0	58.0	58.0	552 bp	0.5	60.9
psbG-ndhC_p5	TACCATAAAGAAGCGGCCAC	TCGATGTACTGGGGGTATCC	50.0	55.0	58.0	58.0	292 bp	0.25	49.7