Hotspot

trnS-trnT

LSC · 7826-8451 · 626 bp

Back to Ricotia

Pi 0.0456

MarkerSeek score 80.50

Primer available yes

Label trnS-trnT

Feature Metadata

label_name: trnS-trnT
feature_type: igs
region: LSC
length_bp: 626
variable_sites: 53
pi: 0.0456
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 80.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-trnT_p1	CCAACGCTTTAGTCCACTCA	TACCACTAAACTATACCCGC	50.0	45.0	57.8	54.0	677-704 bp	1.0	55.2
trnS-trnT_p2	AACGCTTTAGTCCACTCAGC	TACCACTAAACTATACCCGC	50.0	45.0	58.2	54.0	675-702 bp	1.0	54.9
trnS-trnT_p3	CCAACGCTTTAGTCCACTCA	TTACCACTAAACTATACCCGC	50.0	42.9	57.8	54.9	678-705 bp	1.0	54.1
trnS-trnT_p4	AGAACGGGAAAGAGAGGGAT	TACCACTAAACTATACCCGC	50.0	45.0	57.7	54.0	739-766 bp	1.0	53.8
trnS-trnT_p5	AACGCTTTAGTCCACTCAGC	TTACCACTAAACTATACCCGC	50.0	42.9	58.2	54.9	676-703 bp	1.0	53.8