Hotspot

ycf1

SSC · 127008-132506 · 5499 bp

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Pi 0.0124
MarkerSeek score 51.00
Primer available yes
Label ycf1

Feature Metadata

label_name
ycf1
feature_type
gene
region
SSC
length_bp
5499
variable_sites
275
pi
0.0124
unique_haplotype_count
13
primer_available
yes
alignment_reliability
1.00
markerseek_score
51.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf1_p1 GTTGTTCCCATTGCAAAATAC GCAAGACATCAAAAGATAACTGGA 38.1 37.5 54.3 57.5 171 bp 1.0 47.5
ycf1_p2 GTTGTTCCCATTGCAAAATAC GCAAGACATCAAAAGATAACTGG 38.1 39.1 54.3 56.2 171 bp 1.0 47.5
ycf1_p3 TTGTTCCCATTGCAAAATAC TGCAAGACATCAAAAGATAACTGG 35.0 37.5 52.6 57.7 171 bp 1.0 47.5
ycf1_p4 GTTGTTCCCATTGCAAAATAC TGCAAGACATCAAAAGATAACTGG 38.1 37.5 54.3 57.7 172 bp 1.0 47.4
ycf1_p5 GTTGTTCCCATTGCAAAATA TGCAAGACATCAAAAGATAACTGG 35.0 37.5 52.6 57.7 172 bp 1.0 47.4