Hotspot

rpl32-trnL-UAG

SSC · 116551-117434 · 884 bp

Back to Ribes

Pi 0.0265
MarkerSeek score 68.50
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
884
variable_sites
87
pi
0.0265
unique_haplotype_count
13
primer_available
yes
alignment_reliability
0.99
markerseek_score
68.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 GGGATATTGGGCAGCGTTAA AGCGTGTCTACCGATTTCAC 50.0 50.0 58.0 58.0 820-1015 bp 1.0 91.2
rpl32-trnL-UAG_p2 GGGATATTGGGCAGCGTTAA CAGCGTGTCTACCGATTTCA 50.0 50.0 58.0 58.0 821-1016 bp 1.0 91.2
rpl32-trnL-UAG_p3 GGGATATTGGGCAGCGTTAA TTTAGAAGATCCCATGCCGC 50.0 50.0 58.0 58.0 885-1080 bp 1.0 90.7
rpl32-trnL-UAG_p4 GGGATATTGGGCAGCGTTAA AGTCCTGCGATTTCATCCAC 50.0 50.0 58.0 58.0 1085-1280 bp 1.0 90.4
rpl32-trnL-UAG_p5 GGGATATTGGGCAGCGTTAA GTCCTGCGATTTCATCCACT 50.0 50.0 58.0 58.0 1084-1279 bp 1.0 90.4