Hotspot

psbE-petL

LSC · 67558-68865 · 1308 bp

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Pi 0.0132
MarkerSeek score 57.80
Primer available yes
Label psbE-petL

Feature Metadata

label_name
psbE-petL
feature_type
igs
region
LSC
length_bp
1308
variable_sites
62
pi
0.0132
unique_haplotype_count
13
primer_available
yes
alignment_reliability
0.99
markerseek_score
57.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbE-petL_p1 ATGGAATTCCTTGTCGGCTC GCCGCTAGTAGAAAACCGAA 50.0 50.0 58.0 58.0 1407-1544 bp 0.8 78.7
psbE-petL_p2 TTCGGGTTTGATTGTGTCGT GCCGCTAGTAGAAAACCGAA 45.0 50.0 58.0 58.0 1637-1774 bp 0.8 78.5
psbE-petL_p3 TCGTTCGGGTTTGATTGTGT GCCGCTAGTAGAAAACCGAA 45.0 50.0 58.0 58.0 1640-1777 bp 0.8 78.4
psbE-petL_p4 TCGTTCGGGTTTGATTGTGT AAGCCGCTAGTAGAAAACCG 45.0 50.0 58.0 58.0 1642-1779 bp 0.8 78.4
psbE-petL_p5 TTCGGGTTTGATTGTGTCGT AAGCCGCTAGTAGAAAACCG 45.0 50.0 58.0 58.0 1639-1776 bp 0.8 78.4