Hotspot

ycf4-cemA

LSC · 63561-64153 · 593 bp

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Pi 0.0114
MarkerSeek score 61.10
Primer available yes
Label ycf4-cemA

Feature Metadata

label_name
ycf4-cemA
feature_type
igs
region
LSC
length_bp
593
variable_sites
27
pi
0.0114
unique_haplotype_count
13
primer_available
yes
alignment_reliability
1.00
markerseek_score
61.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf4-cemA_p1 CGTTCCCTTGACTCGTACTG AGAGAGATCCACCAAGGCAA 55.0 50.0 58.0 58.3 758-774 bp 1.0 82.6
ycf4-cemA_p2 TTGCGTGTACCGATTGAAGT AGAGAGATCCACCAAGGCAA 45.0 50.0 57.8 58.3 682-698 bp 1.0 82.0
ycf4-cemA_p3 CGTTCCCTTGACTCGTACTG GAGAGATCCACCAAGGCAAA 55.0 50.0 58.0 57.5 757-773 bp 1.0 81.4
ycf4-cemA_p4 CGTTCCCTTGACTCGTACTG AGAGAGAGATCCACCAAGGC 55.0 55.0 58.0 58.5 760-776 bp 1.0 81.3
ycf4-cemA_p5 AAAAGCTGCGGAATTAGCCT AGAGAGATCCACCAAGGCAA 45.0 50.0 58.2 58.3 707-723 bp 0.933333 79.0