Hotspot

trnF-GAA-ndhJ

LSC · 51290-51959 · 670 bp

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Pi 0.0179
MarkerSeek score 64.10
Primer available yes
Label trnF-GAA-ndhJ

Feature Metadata

label_name
trnF-GAA-ndhJ
feature_type
igs
region
LSC
length_bp
670
variable_sites
44
pi
0.0179
unique_haplotype_count
12
primer_available
yes
alignment_reliability
1.00
markerseek_score
64.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnF-GAA-ndhJ_p1 CCTCGTGTCACCAGTTCAAA ATCCACGCCTGAAACGTATC 50.0 50.0 58.1 58.1 789-814 bp 1.0 87.4
trnF-GAA-ndhJ_p2 CCTCGTGTCACCAGTTCAAA AGTTGGATAGGATGGCCCTT 50.0 50.0 58.1 58.1 757-782 bp 1.0 87.4
trnF-GAA-ndhJ_p3 CCTCGTGTCACCAGTTCAAA CCACGCCTGAAACGTATCTT 50.0 50.0 58.1 58.3 787-812 bp 1.0 85.7
trnF-GAA-ndhJ_p4 CCTCGTGTCACCAGTTCAAA CATCCACGCCTGAAACGTAT 50.0 50.0 58.1 58.3 790-815 bp 1.0 85.2
trnF-GAA-ndhJ_p5 TCGGGATAGCTCAGTTGGTA ATCCACGCCTGAAACGTATC 50.0 50.0 57.6 58.1 827-852 bp 1.0 84.0