Hotspot

psbZ-trnG-UCC

LSC · 38415-38981 · 567 bp

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Pi 0.0224
MarkerSeek score 66.50
Primer available yes
Label psbZ-trnG-UCC

Feature Metadata

label_name
psbZ-trnG-UCC
feature_type
igs
region
LSC
length_bp
567
variable_sites
47
pi
0.0224
unique_haplotype_count
12
primer_available
yes
alignment_reliability
0.98
markerseek_score
66.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbZ-trnG-UCC_p1 CCTGATGGTTGGTCAAGTAA CGTCTTCTCCTTGGCAAAGA 45.0 50.0 55.0 57.8 690-746 bp 1.0 63.7
psbZ-trnG-UCC_p2 TCCGGTACATCATTATGGATTGG CGTCTTCTCCTTGGCAAAGA 43.5 50.0 58.1 57.8 654-710 bp 1.0 63.6
psbZ-trnG-UCC_p3 CCGGTACATCATTATGGATTGG CGTCTTCTCCTTGGCAAAGA 45.5 50.0 56.7 57.8 653-709 bp 1.0 61.9
psbZ-trnG-UCC_p4 CCTGATGGTTGGTCAAGTAAT CGTCTTCTCCTTGGCAAAGA 42.9 50.0 55.6 57.8 690-746 bp 1.0 60.5
psbZ-trnG-UCC_p5 CCGGTACATCATTATGGATTGGA CGTCTTCTCCTTGGCAAAGA 43.5 50.0 58.1 57.8 653-709 bp 0.933333 60.3