Hotspot

psbM-trnD-GUC

LSC · 31530-32685 · 1156 bp

Back to Ribes

Pi 0.0171
MarkerSeek score 65.30
Primer available yes
Label psbM-trnD-GUC

Feature Metadata

label_name
psbM-trnD-GUC
feature_type
igs
region
LSC
length_bp
1156
variable_sites
79
pi
0.0171
unique_haplotype_count
13
primer_available
yes
alignment_reliability
1.00
markerseek_score
65.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbM-trnD-GUC_p1 AGAATGAACAGTGCAGTAGC TTCAATTGGTCAGAGCACCG 45.0 50.0 55.8 58.5 1264-1277 bp 1.0 66.3
psbM-trnD-GUC_p2 AGGAACTAGAATGAACAGTGCA TTCAATTGGTCAGAGCACCG 40.9 50.0 57.4 58.5 1271-1284 bp 1.0 63.1
psbM-trnD-GUC_p3 AGAATGAACAGTGCAGTAGC GTTCAATTGGTCAGAGCACC 45.0 50.0 55.8 57.0 1265-1278 bp 1.0 62.1
psbM-trnD-GUC_p4 GGAACTAGAATGAACAGTGCAG TTCAATTGGTCAGAGCACCG 45.5 50.0 57.1 58.5 1270-1283 bp 1.0 60.6
psbM-trnD-GUC_p5 AGAATGAACAGTGCAGTAGC TCAATTGGTCAGAGCACCG 45.0 52.6 55.8 57.8 1263-1276 bp 1.0 60.3