Hotspot

petN-psbM

LSC · 30237-31424 · 1188 bp

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Pi 0.0163
MarkerSeek score 60.40
Primer available yes
Label petN-psbM

Feature Metadata

label_name
petN-psbM
feature_type
igs
region
LSC
length_bp
1188
variable_sites
72
pi
0.0163
unique_haplotype_count
13
primer_available
yes
alignment_reliability
1.00
markerseek_score
60.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petN-psbM_p1 GCGAGTCTCTGAATGCTTGA AGGATCCTGCCCCTCTTATC 50.0 55.0 58.0 58.0 2260-2535 bp 1.0 88.0
petN-psbM_p2 GCGAGTCTCTGAATGCTTGA CCGTCCTTTTGTCCACTTCA 50.0 50.0 58.0 58.0 2052-2338 bp 1.0 87.9
petN-psbM_p3 TCCAAGGGTGTTACTGCCTA AGGATCCTGCCCCTCTTATC 50.0 55.0 58.0 58.0 1950-2223 bp 1.0 87.8
petN-psbM_p4 TCCAAGGGTGTTACTGCCTA CCGTCCTTTTGTCCACTTCA 50.0 50.0 58.0 58.0 1742-2026 bp 1.0 87.7
petN-psbM_p5 GCGAGTCTCTGAATGCTTGA ACGGAAGGCGGTAGGTTATA 50.0 50.0 58.0 57.9 1952-2238 bp 1.0 87.7