Hotspot

trnS-GCU-trnG-GCC

LSC · 9230-10109 · 880 bp

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Pi 0.0189
MarkerSeek score 65.50
Primer available yes
Label trnS-GCU-trnG-GCC

Feature Metadata

label_name
trnS-GCU-trnG-GCC
feature_type
igs
region
LSC
length_bp
880
variable_sites
66
pi
0.0189
unique_haplotype_count
12
primer_available
yes
alignment_reliability
0.99
markerseek_score
65.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GCU-trnG-GCC_p1 CAATCCGACGCTTTAGTCCA GGACCCCTTAACTATTAAAGGGG 50.0 47.8 58.0 57.9 721-1006 bp 1.0 65.0
trnS-GCU-trnG-GCC_p2 AATCCGACGCTTTAGTCCAC GGACCCCTTAACTATTAAAGGGG 50.0 47.8 58.0 57.9 720-1005 bp 1.0 65.0
trnS-GCU-trnG-GCC_p3 ACGGAAAGAGAGGGATTCGA GGACCCCTTAACTATTAAAGGGG 50.0 47.8 58.2 57.9 775-1060 bp 1.0 63.5
trnS-GCU-trnG-GCC_p4 AGCAATCCGACGCTTTAGTC GGACCCCTTAACTATTAAAGGGG 50.0 47.8 58.4 57.9 723-1008 bp 1.0 62.2
trnS-GCU-trnG-GCC_p5 CGACGCTTTAGTCCACTCAG GGACCCCTTAACTATTAAAGGGG 55.0 47.8 58.4 57.9 716-1001 bp 1.0 62.0