Hotspot

trnK-UUU-rps16

LSC · 4311-5157 · 847 bp

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Pi 0.0186
MarkerSeek score 64.30
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
847
variable_sites
67
pi
0.0186
unique_haplotype_count
13
primer_available
yes
alignment_reliability
0.97
markerseek_score
64.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 CCCTTTCAGGATCAGTCGTG AAAAGGGTATGTTGCTGCCA 55.0 45.0 58.0 58.0 1594-1672 bp 0.933333 84.9
trnK-UUU-rps16_p2 CCTTTCAGGATCAGTCGTGG AAAAGGGTATGTTGCTGCCA 55.0 45.0 58.0 58.0 1593-1671 bp 0.933333 84.9
trnK-UUU-rps16_p3 CGAATCCCTTGCTTCATCCA AAAAGGGTATGTTGCTGCCA 50.0 45.0 58.0 58.0 1546-1624 bp 0.933333 84.7
trnK-UUU-rps16_p4 ACGAATCCCTTGCTTCATCC AAAAGGGTATGTTGCTGCCA 50.0 45.0 58.0 58.0 1547-1625 bp 0.933333 84.7
trnK-UUU-rps16_p5 GGACGAATCCCTTGCTTCAT AAAAGGGTATGTTGCTGCCA 50.0 45.0 58.0 58.0 1549-1627 bp 0.933333 84.7