Hotspot

psbC

LSC · 34881-36302 · 1422 bp

Back to Rhynchotechum

Pi 0.0113

MarkerSeek score 55.80

Primer available yes

Label psbC

Feature Metadata

label_name: psbC
feature_type: gene
region: LSC
length_bp: 1422
variable_sites: 16
pi: 0.0113
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbC_p1	AGGTATTCGTGCTTGGATGG	ACATTGGATCCCCTGTCTCA	50.0	50.0	57.7	58.0	1480-1482 bp	1.0	78.2
psbC_p2	TATTCGTGCTTGGATGGCG	ACATTGGATCCCCTGTCTCA	52.6	50.0	58.3	58.0	1477-1479 bp	1.0	70.4
psbC_p3	GGTATTCGTGCTTGGATGGC	ACATTGGATCCCCTGTCTCA	55.0	50.0	59.3	58.0	1479-1481 bp	1.0	70.2
psbC_p4	AAGGTATTCGTGCTTGGATGG	ACATTGGATCCCCTGTCTCA	47.6	50.0	58.4	58.0	1481-1483 bp	1.0	70.0
psbC_p5	GATGGCGGCTCAAGATCAG	ACATTGGATCCCCTGTCTCA	57.9	50.0	58.4	58.0	1465-1467 bp	1.0	69.8