Hotspot

cemA

LSC · 62897-63589 · 693 bp

Back to Rhynchotechum

Pi 0.00144

MarkerSeek score 56.40

Primer available yes

Label cemA

Feature Metadata

label_name: cemA
feature_type: gene
region: LSC
length_bp: 693
variable_sites: 1
pi: 0.00144
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
cemA_p1	GTCGACGAATGAAGTGGGTT	ATGAAAATCCCCCGGGTAGA	50.0	50.0	58.2	58.1	833 bp	1.0	77.6
cemA_p2	GAGTCGACGAATGAAGTGGG	ATGAAAATCCCCCGGGTAGA	55.0	50.0	58.4	58.1	835 bp	1.0	76.4
cemA_p3	GTCGACGAATGAAGTGGGTT	GATGAAAATCCCCCGGGTAG	50.0	55.0	58.2	57.7	834 bp	1.0	76.3
cemA_p4	GTCGACGAATGAAGTGGGTT	CCCCCGGGTAGAAAGTTTTT	50.0	50.0	58.2	57.7	825 bp	1.0	76.1
cemA_p5	GTCGACGAATGAAGTGGGTT	ATCCCCCGGGTAGAAAGTTT	50.0	50.0	58.2	58.3	827 bp	1.0	75.9