Hotspot

ndhD-psaC

SSC · 117819-117910 · 92 bp

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Pi 0.0217

MarkerSeek score 65.00

Primer available yes

Label ndhD-psaC

Feature Metadata

label_name: ndhD-psaC
feature_type: igs
region: SSC
length_bp: 92
variable_sites: 2
pi: 0.0217
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD-psaC_p1	AACCCGCAGATATTGGCAAA	GACCGAAGACTGTGTTGGTT	45.0	50.0	58.2	58.1	289 bp	1.0	78.9
ndhD-psaC_p2	ACCCGCAGATATTGGCAAAA	GACCGAAGACTGTGTTGGTT	45.0	50.0	58.2	58.1	288 bp	1.0	78.9
ndhD-psaC_p3	AACCCGCAGATATTGGCAAA	CCGACCGATTTCTTGAGTGT	45.0	50.0	58.2	57.9	246 bp	1.0	78.8
ndhD-psaC_p4	ACCCGCAGATATTGGCAAAA	CCGACCGATTTCTTGAGTGT	45.0	50.0	58.2	57.9	245 bp	1.0	78.8
ndhD-psaC_p5	CCCGCAGATATTGGCAAAAC	GACCGAAGACTGTGTTGGTT	50.0	50.0	57.8	58.1	287 bp	1.0	78.5