Hotspot

ccsA-ndhD

SSC · 116046-116288 · 243 bp

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Pi 0.0537
MarkerSeek score 76.80
Primer available yes
Label ccsA-ndhD

Feature Metadata

label_name
ccsA-ndhD
feature_type
igs
region
SSC
length_bp
243
variable_sites
13
pi
0.0537
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
76.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ccsA-ndhD_p1 TTCAGCAATTGTGGCGTCTA ACCCGTACTAAGTATTGGTATGT 45.0 39.1 57.8 56.5 451-452 bp 1.0 45.4
ccsA-ndhD_p2 GCAATTGTGGCGTCTATTGG ACCCGTACTAAGTATTGGTATGT 50.0 39.1 57.8 56.5 447-448 bp 1.0 45.3
ccsA-ndhD_p3 TCAGCAATTGTGGCGTCTAT ACCCGTACTAAGTATTGGTATGT 45.0 39.1 57.6 56.5 450-451 bp 1.0 43.6
ccsA-ndhD_p4 TTCAGCAATTGTGGCGTCTA TCAGTTGAAAAGGTTGAAGT 45.0 35.0 57.8 52.8 405-406 bp 1.0 42.8
ccsA-ndhD_p5 GCAATTGTGGCGTCTATTGG TCAGTTGAAAAGGTTGAAGT 50.0 35.0 57.8 52.8 401-402 bp 1.0 42.8