Hotspot

ycf4-cemA

LSC · 62027-62896 · 870 bp

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Pi 0.0069

MarkerSeek score 62.70

Primer available yes

Label ycf4-cemA

Feature Metadata

label_name: ycf4-cemA
feature_type: igs
region: LSC
length_bp: 870
variable_sites: 6
pi: 0.0069
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf4-cemA_p1	CGTTGACCCGTACTGATGAG	GAGAGAGAAATCCAACAGGACA	55.0	45.5	58.1	57.5	1045 bp	1.0	59.4
ycf4-cemA_p2	CGTTGACCCGTACTGATGAG	TGAGAGAGAAATCCAACAGGAC	55.0	45.5	58.1	57.5	1046 bp	1.0	59.4
ycf4-cemA_p3	ACAAAAAGCCGCTGAATTGG	GAGAGAGAAATCCAACAGGACA	45.0	45.5	57.8	57.5	1002 bp	1.0	58.9
ycf4-cemA_p4	ACAAAAAGCCGCTGAATTGG	TGAGAGAGAAATCCAACAGGAC	45.0	45.5	57.8	57.5	1003 bp	1.0	58.9
ycf4-cemA_p5	CGTTGACCCGTACTGATGAG	TCCAACAGGACAAAAACACT	55.0	40.0	58.1	55.3	1035 bp	1.0	58.2