Hotspot

rpoC1-trnC-GCA

LSC · 24220-28618 · 4399 bp

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Pi 0.00501
MarkerSeek score 51.50
Primer available yes
Label rpoC1-trnC-GCA

Feature Metadata

label_name
rpoC1-trnC-GCA
feature_type
igs
region
LSC
length_bp
4399
variable_sites
22
pi
0.00501
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
51.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpoC1-trnC-GCA_p1 ACCCAGTTCCAACAATGCAT ATTCAGGAAGAACAGGTGGT 45.0 45.0 58.0 56.3 229 bp 1.0 69.8
rpoC1-trnC-GCA_p2 AACTGCCTGACGTTGCATAT ATTCAGGAAGAACAGGTGGT 45.0 45.0 57.9 56.3 189 bp 1.0 69.7
rpoC1-trnC-GCA_p3 ACTGCCTGACGTTGCATATT ATTCAGGAAGAACAGGTGGT 45.0 45.0 57.9 56.3 188 bp 1.0 69.7
rpoC1-trnC-GCA_p4 CAACCCAGTTCCAACAATGC ATTCAGGAAGAACAGGTGGT 50.0 45.0 57.8 56.3 231 bp 1.0 68.7
rpoC1-trnC-GCA_p5 AACCCAGTTCCAACAATGCA ATTCAGGAAGAACAGGTGGT 45.0 45.0 58.2 56.3 230 bp 1.0 68.2