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atpH-atpI

LSC · 14103-15164 · 1062 bp

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Pi 0.00568

MarkerSeek score 62.60

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 1062
variable_sites: 6
pi: 0.00568
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 62.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	TCCGATAGAAGCAAGCCCTA	TCCCTGTCATGTTCCTTGGA	50.0	50.0	57.9	58.3	1232-1235 bp	1.0	78.0
atpH-atpI_p2	AACGGAAGCAGCAGAAATCA	TCCCTGTCATGTTCCTTGGA	45.0	50.0	58.1	58.3	1193-1196 bp	1.0	77.9
atpH-atpI_p3	TACCTTGACCAACTCCAGGT	TCCCTGTCATGTTCCTTGGA	50.0	50.0	57.9	58.3	1251-1254 bp	1.0	77.8
atpH-atpI_p4	TCCAGGTCCGATAGAAGCAA	TCCCTGTCATGTTCCTTGGA	50.0	50.0	58.1	58.3	1238-1241 bp	1.0	77.6
atpH-atpI_p5	GATAGAAGCAAGCCCTACGG	TCCCTGTCATGTTCCTTGGA	55.0	50.0	57.8	58.3	1229-1232 bp	1.0	77.5