Hotspot

rpl22

LSC · 87208-87693 · 486 bp

Back to Rhus

Pi 0.0152
MarkerSeek score 48.80
Primer available yes
Label rpl22

Feature Metadata

label_name
rpl22
feature_type
gene
region
LSC
length_bp
486
variable_sites
20
pi
0.0152
unique_haplotype_count
7
primer_available
yes
alignment_reliability
1.00
markerseek_score
48.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl22_p1 TTTGGCTGTGCAAACCAAAG GGAAGGGCTCCAATTGGTAG 45.0 55.0 58.0 57.9 788-1087 bp 1.0 87.0
rpl22_p2 TTTGGCTGTGCAAACCAAAG CCGGATCGAAATGTTGGCTA 45.0 50.0 58.0 58.1 874-1173 bp 1.0 86.7
rpl22_p3 GGTCTATTTCCATGGCTGGG GCCGCCGTAGTAAATAGGAG 55.0 55.0 58.0 57.9 1306-1599 bp 0.75 75.9
rpl22_p4 GGTCTATTTCCATGGCTGGG GGAAGGGCTCCAATTGGTAG 55.0 55.0 58.0 57.9 1412-1705 bp 0.75 75.8
rpl22_p5 GGTCTATTTCCATGGCTGGG CCGGATCGAAATGTTGGCTA 55.0 50.0 58.0 58.1 1498-1791 bp 0.75 75.7