Hotspot

rpl2

IRa · 158339-159828 · 1490 bp

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Pi 0.000959
MarkerSeek score 35.80
Primer available yes
Label rpl2

Feature Metadata

label_name
rpl2
feature_type
gene
region
IRa
length_bp
1490
variable_sites
5
pi
0.000959
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
35.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl2_p1 GAATGGTTAAAGCGCCCAAC AGTGAACGTGGCATAGTAAA 50.0 40.0 58.0 54.7 2108-2110 bp 0.5 33.8
rpl2_p2 GAATGGTTAAAGCGCCCAAC TTTAGTGAACGTGGCATAGT 50.0 40.0 58.0 54.7 2111-2113 bp 0.5 33.8
rpl2_p3 AGCATTGGGTCGAACTCTTC AGTGAACGTGGCATAGTAAA 50.0 40.0 57.9 54.7 1775-1777 bp 0.5 33.1
rpl2_p4 GCATTGGGTCGAACTCTTCT AGTGAACGTGGCATAGTAAA 50.0 40.0 57.9 54.7 1774-1776 bp 0.5 33.1
rpl2_p5 GCATTGGGTCGAACTCTTCT TTTAGTGAACGTGGCATAGT 50.0 40.0 57.9 54.7 1777-1779 bp 0.5 33.1