Hotspot

ndhD

SSC · 120490-121992 · 1503 bp

Pi 0.00898

MarkerSeek score 47.90

Primer available yes

Label ndhD

Feature Metadata

label_name: ndhD
feature_type: gene
region: SSC
length_bp: 1503
variable_sites: 39
pi: 0.00898
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 47.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD_p1	TGGATATGCTACTTCGGGGT	TCTGCTCCAAGAACAGAGGA	50.0	50.0	57.9	58.0	2160-2243 bp	1.0	83.7
ndhD_p2	TGTGGCTTCTATGGGCTTTC	TCTGCTCCAAGAACAGAGGA	50.0	50.0	57.9	58.0	2188-2271 bp	1.0	83.4
ndhD_p3	TGGATATGCTACTTCGGGGT	CTGCTCCAAGAACAGAGGAC	50.0	55.0	57.9	57.9	2159-2242 bp	1.0	83.1
ndhD_p4	GCTAATGAGGCATGGGGATC	TCTGCTCCAAGAACAGAGGA	55.0	50.0	58.2	58.0	2319-2402 bp	1.0	83.0
ndhD_p5	ACAAACATAGGACAGCGCAA	TCTGCTCCAAGAACAGAGGA	45.0	50.0	58.1	58.0	2047-2108 bp	0.75	73.0