Hotspot

ccsA-ndhD

SSC · 120139-120489 · 351 bp

Back to Rhus

Pi 0.0375
MarkerSeek score 72.70
Primer available yes
Label ccsA-ndhD

Feature Metadata

label_name
ccsA-ndhD
feature_type
igs
region
SSC
length_bp
351
variable_sites
29
pi
0.0375
unique_haplotype_count
8
primer_available
yes
alignment_reliability
0.96
markerseek_score
72.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ccsA-ndhD_p1 AGCGCTATTTTTACCCAGGG TTATCGATGGCTTCCCTTGC 50.0 50.0 57.9 58.0 1318-1402 bp 1.0 85.3
ccsA-ndhD_p2 AGCGCTATTTTTACCCAGGG CGCTGCACTCTTTTTCTTGG 50.0 50.0 57.9 58.0 1433-1517 bp 1.0 84.9
ccsA-ndhD_p3 AGCGCTATTTTTACCCAGGG TATCTTGACGAAATGGGCGG 50.0 50.0 57.9 58.1 1381-1465 bp 1.0 84.9
ccsA-ndhD_p4 AGCGCTATTTTTACCCAGGG ATGGGTTTATTAGCGCTGCA 50.0 45.0 57.9 57.9 1446-1530 bp 1.0 84.8
ccsA-ndhD_p5 AGCGCTATTTTTACCCAGGG TGACGAAATGGGCGGATTAG 50.0 50.0 57.9 58.1 1429-1442 bp 0.25 49.9