Hotspot

ndhF-rpl32

SSC · 116728-117690 · 963 bp

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Pi 0.0238
MarkerSeek score 67.70
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
963
variable_sites
65
pi
0.0238
unique_haplotype_count
8
primer_available
yes
alignment_reliability
0.97
markerseek_score
67.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 CTGGAAGTGGAACGAAAGGT CGTGGAAAGGGATTTCGCTA 50.0 50.0 57.7 58.0 1135-1507 bp 0.75 74.4
ndhF-rpl32_p2 CTTTTTGACAAGCACTGGCC CGTGGAAAGGGATTTCGCTA 50.0 50.0 58.1 58.0 1615-1987 bp 0.75 73.9
ndhF-rpl32_p3 CTGGAAGTGGAACGAAAGGT AGTGCTTTCCCTTTTGGAGG 50.0 50.0 57.7 58.0 1628-2034 bp 0.625 73.8
ndhF-rpl32_p4 CTTTTTGACAAGCACTGGCC AGTGCTTTCCCTTTTGGAGG 50.0 50.0 58.1 58.0 2108-2514 bp 0.625 73.6
ndhF-rpl32_p5 CTTTTTGACAAGCACTGGCC GGTGGGGTGATGGGAAAAAT 50.0 50.0 58.1 58.0 1881-1963 bp 0.5 65.4