Hotspot

trnG-UCC-trnR-UCU

LSC · 11324-11507 · 184 bp

Back to Rhus

Pi 0.0289
MarkerSeek score 56.30
Primer available yes
Label trnG-UCC-trnR-UCU

Feature Metadata

label_name
trnG-UCC-trnR-UCU
feature_type
igs
region
LSC
length_bp
184
variable_sites
10
pi
0.0289
unique_haplotype_count
6
primer_available
yes
alignment_reliability
0.96
markerseek_score
56.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnG-UCC-trnR-UCU_p1 AGCCTTCCAAGCTAACGATG AGAAGACCTCTGTCCTATCCA 50.0 47.6 58.0 56.9 256-264 bp 1.0 68.9
trnG-UCC-trnR-UCU_p2 AGCCTTCCAAGCTAACGATG CCCAAGGTTTAGAAGACCTCTG 50.0 50.0 58.0 58.1 266-274 bp 1.0 68.7
trnG-UCC-trnR-UCU_p3 CCTAGCCTTCCAAGCTAACG AGAAGACCTCTGTCCTATCCA 55.0 47.6 58.1 56.9 259-267 bp 1.0 68.7
trnG-UCC-trnR-UCU_p4 AGCCTTCCAAGCTAACGATG ACCCAAGGTTTAGAAGACCTC 50.0 47.6 58.0 56.9 267-275 bp 1.0 68.6
trnG-UCC-trnR-UCU_p5 CCTAGCCTTCCAAGCTAACG CCCAAGGTTTAGAAGACCTCTG 55.0 50.0 58.1 58.1 269-277 bp 1.0 68.4